Effects of Sodium Azide on the Abundance of Prokaryotes and Viruses in Marine Samples
- Author(s)
- Christian Winter, Marie-Emmanuelle Kerros, Markus Weinbauer
- Abstract
Flow cytometry is set to become the standard method for enumerating prokaryotes and viruses in marine samples. However, the samples need to be flash-frozen in liquid nitrogen directly after aldehyde fixation. Because liquid nitrogen may not always be available, we tested the potential of sodium azide as a preservative for prokaryotes and viruses in marine samples as a possible alternative. For that we conducted incubation experiments with untreated and sodium azide treated marine water samples at 4 degrees C and room temperature. The data indicate that sodium azide cannot be used to maintain marine samples used for the enumeration of prokaryotes and viruses.
- Organisation(s)
- Functional and Evolutionary Ecology
- External organisation(s)
- Laboratoire d'Océanographie de Villefranche
- Journal
- PLoS ONE
- Volume
- 7
- ISSN
- 1932-6203
- DOI
- https://doi.org/10.1371/journal.pone.0037597
- Publication date
- 2012
- Peer reviewed
- Yes
- Austrian Fields of Science 2012
- 106022 Microbiology
- Sustainable Development Goals
- SDG 14 - Life Below Water
- Portal url
- https://ucris.univie.ac.at/portal/en/publications/effects-of-sodium-azide-on-the-abundance-of-prokaryotes-and-viruses-in-marine-samples(d0bcc411-289e-4a85-a09c-f50a745fcea8).html