Major role of nitrite-oxidizing bacteria in dark ocean carbon fixation

Author(s)
Maria G. Pachiadaki, Eva Sintes, Kristin Bergauer, Julia M. Brown, Nicholas R. Record, Brandon K. Swan, Mary Elizabeth Mathyer, Steven J. Hallam, Purificacion Lopez-Garcia, Yoshihiro Takaki, Takuro Nunoura, Tanja Woyke, Gerhard J. Herndl, Ramunas Stepanauskas
Abstract

Carbon fixation by chemoautotrophic microorganisms in the dark ocean has a major impact on global carbon cycling and ecological relationships in the ocean's interior, but the relevant taxa and energy sources remain enigmatic. We show evidence that nitrite-oxidizing bacteria affiliated with the Nitrospinae phylum are important in dark ocean chemoautotrophy. Single-cell genomics and community metagenomics revealed that Nitrospinae are the most abundant and globally distributed nitrite-oxidizing bacteria in the ocean. Metaproteomics and metatranscriptomics analyses suggest that nitrite oxidation is the main pathway of energy production in Nitrospinae. Microautoradiography, linked with catalyzed reporter deposition fluorescence in situ hybridization, indicated that Nitrospinae fix 15 to 45% of inorganic carbon in the mesopelagic western North Atlantic. Nitrite oxidation may have a greater impact on the carbon cycle than previously assumed.

Organisation(s)
Functional and Evolutionary Ecology
External organisation(s)
Bigelow Laboratory for Ocean Sciences, National Biodefense Analysis and Countermeasures Center, Washington University in St. Louis, University of British Columbia (UBC), Université Paris XI - Paris-Sud, Université Paris-Saclay, Centre National de la Recherche Scientifique (CNRS), Japan Agency for Marine-Earth Science and Technology, U.S. Department of Energy Joint Genome Institute, Royal Netherlands Institute for Sea Research, Utrecht University
Journal
Science
Volume
358
Pages
1046-1050
No. of pages
5
ISSN
0036-8075
DOI
https://doi.org/10.1126/science.aan8260
Publication date
11-2017
Peer reviewed
Yes
Austrian Fields of Science 2012
Marine biology
Keywords
Portal url
https://ucris.univie.ac.at/portal/en/publications/major-role-of-nitriteoxidizing-bacteria-in-dark-ocean-carbon-fixation(09702c18-f7e4-498a-8172-53dfdc519a1e).html